Jove
Visualize
Contact Us

Related Experiment Videos

Peas (Pisum sativum L.).

Jan Grant1, Pauline Cooper

  • 1New Zealand for Crop & Food Research, Ltd., Christchurch, New Zealand.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of source-to-sink transport of methionine in establishing seed protein quantity and quality in legumes.

Plant physiology·2021
Same author

Manipulation of sucrose phloem and embryo loading affects pea leaf metabolism, carbon and nitrogen partitioning to sinks as well as seed storage pools.

The Plant journal : for cell and molecular biology·2019
Same author

Expression of Genes Related to Sugar and Amino Acid Transport and Cytokinin Metabolism during Leaf Development and Senescence in <i>Pisum sativum</i> L.

Plants (Basel, Switzerland)·2019
Same author

A hidden heritage.

Nursing management (Harrow, London, England : 1994)·2016
Same author

Improvement of pea biomass and seed productivity by simultaneous increase of phloem and embryo loading with amino acids.

The Plant journal : for cell and molecular biology·2014
Same author

Developing psychotherapists' competence through clinical supervision: protocol for a qualitative study of supervisory dyads.

BMC psychiatry·2013
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

This study details a reliable method for creating transgenic pea (Pisum sativum) lines. The robust Agrobacterium-mediated transformation technique yields efficient gene transfer for crop improvement.

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Agricultural Science

Background:

  • Developing transgenic pea (Pisum sativum) lines is crucial for crop improvement.
  • Efficient and reproducible transformation methods are essential for genetic studies and breeding.

Purpose of the Study:

  • To describe a robust and long-standing method for Agrobacterium-mediated transformation of pea.
  • To report the efficiency and applicability of the method across diverse pea genotypes.

Main Methods:

  • Immature pea cotyledons were used as explants for transformation.
  • Agrobacterium tumefaciens strains AGL1 and KYRT1 were employed.
  • Kanamycin, phosphinothricin, and hygromycin were utilized as selectable markers.

Related Experiment Videos

Main Results:

  • Transgenic lines were produced for over 30 pea genotypes, including cultivars and breeding lines.
  • Transformation efficiency ranged from 0.2% to 13.5% of explants yielding transformed lines.
  • Successful introduction of genes for pest/disease resistance and quality trait alteration was achieved.

Conclusions:

  • The described method provides a robust platform for generating transgenic pea lines.
  • This technique facilitates the introduction of valuable genes for enhancing pea crop characteristics.
  • The method has been consistently successful since 1992, demonstrating its reliability.