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Related Concept Videos

Solvents01:12

Solvents

A solvent is a substance, most often a liquid, that can dissolve other substances. Here, the substance being dissolved is called a solute. When a solvent and a solute combine, they form a solution - a homogenous mixture of both the solvent and the solute. Water is a universal biological solvent. Its polar structure allows it to dissolve many other polar compounds. The ability of water to dissolve is governed by a balance between water molecules binding to each other and binding to the solute.
A...
The Apoplast and Symplast01:46

The Apoplast and Symplast

Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...

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Related Experiment Video

Updated: Jul 19, 2026

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)
06:26

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

Published on: September 7, 2022

Eggplant (Solanum melongena L.).

Joyce Van Eck1, Ada Snyder

  • 1The Boyce Thompson Institute for Plant Research, Inc., Ithaca, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
Summary

Developing disease resistance in eggplant is crucial for crop improvement. This study presents an Agrobacterium-mediated transformation protocol for eggplant, enabling enhanced disease resistance through biotechnological techniques.

Area of Science:

  • Agricultural Science
  • Plant Biotechnology
  • Genetics

Background:

  • Eggplant (Solanum melongena L.) is a vital crop in Asia and Africa, facing significant losses from bacterial and fungal wilts.
  • Conventional breeding for disease resistance is hampered by sexual incompatibilities with wild relatives.
  • A combined approach of conventional breeding and biotechnology is needed for effective eggplant improvement.

Purpose of the Study:

  • To describe a reliable Agrobacterium-mediated transformation protocol for eggplant.
  • To facilitate the development of disease-resistant eggplant varieties.
  • To overcome limitations in traditional breeding methods.

Main Methods:

  • The protocol involves Agrobacterium-mediated transformation using seedling explants (cotyledons, hypocotyls) and leaves.

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Last Updated: Jul 19, 2026

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)
06:26

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Published on: September 7, 2022

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes
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Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes

Published on: December 22, 2013

  • Two eggplant types, a breeding line and 'Black Eggplant', were used.
  • Neomycin phosphotransferase II (nptII) served as the selectable marker gene with kanamycin as the selection agent.

    • Main Results:

    • Successful recovery of transgenic eggplant lines from both tested varieties.
    • In vitro regenerated transformants demonstrated successful acclimation to greenhouse conditions.
    • The protocol provides a viable method for genetic modification of eggplant.

    Conclusions:

    • Agrobacterium-mediated transformation is an effective tool for eggplant genetic improvement.
    • This protocol can aid in developing eggplant cultivars with enhanced resistance to devastating diseases.
    • Biotechnological approaches are essential for overcoming challenges in eggplant crop enhancement.