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Related Experiment Videos

A double nuclear transfer technique for cloning pigs.

Irina A Polejaeva1, Shawn Walker, Keith Campbell

  • 1Advanced Reproductive Technologies, ViaGen, Inc., Austin, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
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This study details a two-round nuclear transfer (NT) technique for improved embryo development. By using fertilized oocytes, this method bypasses artificial activation, potentially increasing success rates in cloned animals.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Animal Cloning

Background:

  • Nuclear transfer (NT) is a key technique in assisted reproduction and cloning.
  • Traditional NT methods often face challenges with activation efficiency and developmental outcomes.
  • Improving the efficiency of NT procedures is crucial for advancing reproductive technologies.

Purpose of the Study:

  • To describe and evaluate a novel two-round nuclear transfer (NT) procedure.
  • To assess the potential of using fertilized oocytes as recipients in NT to enhance developmental success.
  • To circumvent inefficiencies associated with artificial oocyte activation in NT.

Main Methods:

  • The first NT round involves transferring somatic cell nuclei into enucleated oocytes, followed by fusion, activation, and culture.

Related Experiment Videos

  • The second NT round utilizes karyoplasts from day-old NT embryos transferred into in vivo-derived zygotes with pronuclei removed.
  • Fused couplets undergo electrical pulse treatment and are transferred into synchronized recipient gilts.

    • Main Results:

    • The described two-round NT system utilizes fertilized oocytes, avoiding artificial activation steps.
    • This approach aims to improve the efficiency and success rate of NT procedures.
    • The method is designed to promote more successful embryonic and subsequent development.

    Conclusions:

    • The novel two-round nuclear transfer technique offers a promising alternative to conventional methods.
    • Utilizing fertilized oocytes as cytoplast recipients bypasses activation inefficiencies.
    • This improved NT system may lead to enhanced developmental potential in cloned embryos.