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Reagents to measure and manipulate cell functions.

Kenneth A Giuliano1, D Lansing Taylor, Alan S Waggoner

  • 1Cellumen, Inc., Pittsburgh, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
Summary

New reagents for measuring and manipulating cell functions are key to high-content screening assays. Combining these tools enables a systems biology approach for drug discovery and biomedical research.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Drug Discovery

Background:

  • High-content screening assays rely on reagents to measure and manipulate cellular functions.
  • Diverse measurement reagents exist, including biosensors and fluorescent probes.
  • Emerging manipulation reagents like small inhibitory RNAs and gene switches offer new functional controls.

Purpose of the Study:

  • To highlight the critical role of measurement and manipulation reagents in high-content screening.
  • To discuss recent advancements in reagent development for cell function analysis.
  • To emphasize the potential of combining these reagents for systems cell biology.

Main Methods:

  • Review of existing and novel reagents for cell function measurement.
  • Analysis of reagents designed for specific cell function manipulation.
  • Integration of measurement and manipulation reagents in multiplexed assays.

Main Results:

  • Identification of key reagent classes for cell function analysis.
  • Demonstration of complementary roles for measurement and manipulation reagents.
  • Highlighting the synergy when both reagent types are used in living cells.

Conclusions:

  • Reagents are central to advancing high-content screening capabilities.
  • The combined use of measurement and manipulation reagents facilitates systems cell biology.
  • This integrated approach holds significant promise for drug discovery and biomedical research.

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