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Physiological indicators of cell function.

Michael J Ignatius¹, Jeffrey T Hung

¹Molecular Probes/Invitrogen, Eugene, OR, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 22, 2006

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High content screening (HCS) requires advanced cell-based reagents. This review surveys fluorescent dyes for live and fixed cell assays, highlighting ideal characteristics for optimal assay performance and next-generation applications.

Area of Science:

Biotechnology
Cell Biology
Assay Development

Background:

High content screening (HCS) assays necessitate robust cell-based reagents.
A diverse and growing selection of fluorescent physiological indicators is available.
Key characteristics for ideal reagents include selectivity, signal intensity, and cell solubility.

Purpose of the Study:

To survey existing and novel fluorescent reagents for cell-based assays.
To identify essential properties of fluorescent indicators for optimal assay performance.
To inform the development of next-generation HCS assays.

Main Methods:

Literature review of fluorogenic and color-changing dyes.
Analysis of reagent properties for live cell kinetic and fixed end-point assays.

Related Experiment Videos

Survey of newly developed reagents for advanced HCS applications.

Main Results:

A wide variety of fluorescent physiological indicators exist for cell-based assays.
Optimal reagents balance selectivity, signal intensity, and cell solubility for broad platform compatibility.
Emerging reagents offer enhanced capabilities for future HCS assays.

Conclusions:

The selection of appropriate fluorescent reagents is critical for successful HCS assay development.
Reagent properties must align with assay requirements for reliable and reproducible results.
Continued innovation in fluorescent indicator technology will drive advancements in HCS.