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Transgenic Organisms00:53

Transgenic Organisms

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Jul 19, 2026

Generation of Transgenic C. elegans by Biolistic Transformation
10:15

Generation of Transgenic C. elegans by Biolistic Transformation

Published on: August 23, 2010

Creation of transgenic lines using microparticle bombardment methods.

Vida Praitis1

  • 1Department of Biology, Grinnell College, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
Summary

Microparticle bombardment offers new ways to introduce foreign DNA into Caenorhabditis elegans (C. elegans) for gene research. This method provides an alternative to traditional germ-line injection for genetic transformation.

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Last Updated: Jul 19, 2026

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08:26

Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment

Published on: August 2, 2014

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Exogenous DNA introduction is crucial for C. elegans research, including gene expression studies and functional analysis.
  • Germ-line injection was the primary method for C. elegans transformation historically.
  • Alternative methods are needed to improve transformation efficiency and accessibility.

Purpose of the Study:

  • To describe four microparticle bombardment techniques for transforming Caenorhabditis elegans.
  • To evaluate the advantages and disadvantages of microparticle bombardment for C. elegans transformation.
  • To identify successful cotransformation markers and discuss transformation efficiency.

Main Methods:

  • Microparticle bombardment techniques applied to C. elegans.
  • Introduction of exogenous DNA into the germ line of C. elegans.
  • Evaluation of cotransformation markers and transformation efficiency.

Main Results:

  • Four distinct microparticle bombardment methods for C. elegans transformation are detailed.
  • Comparison of microparticle bombardment with traditional germ-line injection is provided.
  • Successful cotransformation markers for microparticle bombardment are listed.

Conclusions:

  • Microparticle bombardment presents a viable alternative for exogenous DNA delivery in C. elegans.
  • Understanding the nuances of these methods can enhance genetic research in C. elegans.
  • Further optimization of microparticle bombardment can improve transformation efficiency and expand its applications.