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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Published on: June 13, 2014
Assembling a novel bifunctional cellulase-xylanase from Thermotoga maritima by end-to-end fusion
Su Young Hong1, Jin Suk Lee, Kye Man Cho
1Division of Applied Life Science, Gyeongsang National University, Chinju, 660-701, Republic of Korea.
Abstract:
An artificial, bifunctional, thermostable cellulase-xylanase enzyme from Thermotoga maritima by gene fusion. The fusion protein exhibited both cellulase and xylanase activity when xynA was fused downstream of cel5C but no activities were shown when xynA was fused upstream of cel5C. The enzyme was optimally active at pH 5.0 and 80 degrees C over 30 min. E. coli expressed the fusion enzyme, with an apparent molecular mass of approximately 152 kDa by carboxymethyl cellulose- and xylan-SDS-PAGE.
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