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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
Published on: February 2, 2013
A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target
Antony P Black1, Louise Jones, Michael Ardern-Jones
1Cutaneous Immunology Group, MRC Human Immunology Unit, University of Oxford, Weatherall Institute of Molecular Medicine, Oxford OX3 9DS, UK. ablack@hammer.imm.ox.ac.uk
Journal of Immunological Methods
|September 23, 2006
Abstract:
There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.
