Related Experiment Video
Updated: Jul 19, 2026
Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches
Published on: June 25, 2020
Lymph flow, shear stress, and lymphocyte velocity in rat mesenteric prenodal lymphatics
J Brandon Dixon1, Steven T Greiner, Anatoliy A Gashev
1Department of Biomedical Engineering, Texas A&M University, College Station, 77843-1114, USA.
Objective:
To measure lymphocyte velocity, lymphatic contraction, and shear stress in phasically contracting lymphatics in situ.
Methods:
A high-speed video system was used to capture multiple contraction cycles in rat mesenteric lymphatic preparations. The images were analyzed to determine fluid velocity, volume flow rate, wall shear stress, and retrograde flow.
Results:
Lymphocyte density and flux varied from 326 to 35,500 cells/microL and 206 to 2,030 cells/min, respectively. Lymphatics contracted phasically, with a mean diameter 91 +/- 9.0 microm and amplitudes of 39%. Lymph velocity varied with the phasic contractions in both direction and magnitude with an average of 0.87 +/- 0.18 and peaks of 2.2-9.0 mm/s. The velocity was approximately 180 degrees out of phase with the contraction cycle. The average lymph flow was 13.95 +/- 5.27 microL/h with transient periods of flow reversal. This resulted in an average shear of 0.64 +/- 0.14 with peaks of 4-12 dynes/cm(2).
Conclusions:
High-speed lymphocyte tracking provided the spatial and temporal resolution to measure lymphocyte flux throughout the phasic contraction. Poiseuille flow was a reasonable model for estimating wall shear stress through most of the phasic contraction cycle of the intervalvular lymphatic segments. Shear rate was low but had large variations in magnitude compared to that seen in blood vessels.
