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Related Experiment Videos

Circular single-stranded RNA replicon in Saccharomyces cerevisiae.

Y Matsumoto1, R Fishel, R B Wickner

  • 1Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1990
PubMed
Summary

Researchers discovered a circular RNA replicon in yeast that replicates independently. This novel RNA element, appearing in specific yeast strains under acetate conditions, shows similarities to animal RNA viruses.

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Contributions to microbiology·2002

Area of Science:

  • * Molecular Biology
  • * Virology
  • * Yeast Genetics

Background:

  • * Circular RNA replicons are known in plants and rarely in animals.
  • * Their presence and function in yeast were previously uncharacterized.

Purpose of the Study:

  • * To identify and characterize a novel circular RNA replicon in yeast.
  • * To investigate its replication mechanism, inheritance, and host interactions.

Main Methods:

  • * Analysis of RNA species in yeast strains under specific growth conditions (acetate medium).
  • * Characterization of the RNA's physical properties (circularity, association with proteins) using gel electrophoresis and electron microscopy.
  • * Genetic analysis to determine inheritance patterns and identify host factors influencing RNA copy number.

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Main Results:

  • * A 20S circular RNA replicon was identified in specific yeast strains, appearing upon transfer to acetate medium.
  • * This replicon exhibits cytoplasmic (non-Mendelian) inheritance and forms a 32S particle with 23-kDa protein subunits.
  • * The yeast 20S RNA is an independent replicon, distinct from host DNA/RNA and known yeast viruses.
  • * Replication occurs via an RNA-RNA pathway, with a 10,000-fold amplification under acetate conditions.
  • * Host antiviral gene products (SKI) reduce the copy number of this 20S RNA.

Conclusions:

  • * Yeast harbors an independent circular RNA replicon, expanding the known hosts for such elements.
  • * The 20S RNA replicon utilizes an RNA-RNA replication strategy and is regulated by host antiviral systems.
  • * Similarities exist between yeast 20S RNA and the hepatitis delta virus, suggesting conserved mechanisms.