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Updated: Jul 19, 2026

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Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
Published on: November 20, 2013
Thioperamide reduces intracellular calcium in mouse brain synaptosomes
Divya Vohora1, Shanthi N Pal, K K Pillai
1Department of Pharmacology, Faculty of Pharmacy, Hamdard University, New Delhi 110062, India. divyavohora@hotmail.com
Summary
Thioperamide (THP), an H3 receptor antagonist, reduces free calcium in mouse brain synaptosomes. This finding suggests a potential neuroprotective role for THP in neuronal function.
Area of Science:
- Neuroscience
- Cell Biology
- Pharmacology
Background:
- Intracellular calcium regulation is crucial for neuronal survival.
- Elevated intracellular calcium levels can lead to cytotoxicity and neuronal death.
- Histamine H3 receptor antagonists are being investigated for neurological benefits.
Purpose of the Study:
- To investigate the effect of thioperamide (THP) on intracellular calcium levels in mouse brain synaptosomes.
- To explore the potential neuroprotective mechanisms of THP.
Main Methods:
- Preparation of mouse brain synaptosomes.
- Measurement of free intracellular calcium concentrations.
- Application of varying concentrations of thioperamide (THP).
Main Results:
- Thioperamide (THP) demonstrated a concentration-dependent reduction in free calcium within mouse brain synaptosomes.
- This effect was observed for the first time experimentally.
Conclusions:
- Thioperamide (THP) modulates intracellular calcium levels in a manner that may counteract excitotoxicity.
- The observed calcium-reducing effect supports recent findings on THP's anticonvulsant and cognition-enhancing properties.
- Thioperamide (THP) shows promise as a neuroprotective agent.

