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Three dimensional electrode array for cell lysis via electroporation.

Kuan-Ying Lu1, Andrew M Wo, Ying-Jie Lo

  • 1Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan.

Biosensors & Bioelectronics
|September 26, 2006
PubMed
Summary

3D cylindrical electrodes significantly enhance cell lysis via electroporation in microfluidic devices. This novel design offers a more uniform electric field, leading to higher lysis efficiency compared to traditional 2D electrodes.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • Conventional microfabricated devices for cell lysis often use 2D planar or Ag/AgCl electrodes.
  • The electric field from 2D electrodes decays exponentially, causing non-uniform force on cell membranes.

Purpose of the Study:

  • To investigate the cell lysis efficacy of 3D cylindrical electrodes via electroporation in microfluidic platforms.
  • To compare the performance of 3D electrodes against conventional 2D designs.

Main Methods:

  • Computational modeling of electric fields for both 2D and 3D electrode geometries.
  • Experimental cell lysis of leukocytes using microfluidic devices with 2D and 3D electrodes.

Main Results:

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  • 3D electrodes demonstrated a higher effective volume ratio for sufficient electric field strength.
  • Experimental results showed simultaneous multi-pores on leukocytes lysed with 3D electrodes, indicating field uniformity.
  • A single row of 3D electrodes achieved a 30% lysis percentage, significantly higher than 8% for 2D electrodes under identical flow conditions.

    • Conclusions:

    • 3D cylindrical electrodes offer superior performance for cell lysis via electroporation in microfluidics.
    • The enhanced uniformity of electric fields generated by 3D designs improves lysis efficacy.
    • This study provides valuable insights for designing more effective microfluidic cell lysis devices.