Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diagnostic Immunohistochemistry: what can go wrong?

Hadi Yaziji1, Todd Barry

  • 1Ancillary Pathways, Miami, FL 33143, USA. yaziji@ancillarypath.com

Advances in Anatomic Pathology
|September 26, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Suitability of Repurposed, Legacy HER2 Immunohistochemistry Assays for the Detection of HER2 Low and Ultralow Expression: Current Limitations and Potential Considerations.

Applied immunohistochemistry & molecular morphology : AIMM·2025
Same author

Primary central nervous system diffuse large B-cell lymphoma with MYC and BCL2 rearrangements associated with deep brain stimulation device.

Virchows Archiv : an international journal of pathology·2024
Same author

Primary histiocytic sarcoma of the clivus with focal extension into central nervous system and neurologic manifestations: First description at an unusual site with an overwhelming and rapid progression.

Clinical neuropathology·2021
Same author

Prognostic Risk Assessment and Prediction of Radiotherapy Benefit for Women with Ductal Carcinoma In Situ (DCIS) of the Breast, in a Randomized Clinical Trial (SweDCIS).

Cancers·2021
Same author

HER2 Testing: Insights From Pathologists' Perspective on Technically Challenging HER2 FISH Cases.

Applied immunohistochemistry & molecular morphology : AIMM·2021
Same author

Validation of a Ductal Carcinoma <i>In Situ</i> Biomarker Profile for Risk of Recurrence after Breast-Conserving Surgery with and without Radiotherapy.

Clinical cancer research : an official journal of the American Association for Cancer Research·2020

Immunohistochemistry (IHC) is crucial in surgical pathology but faces challenges. This review addresses common diagnostic IHC pitfalls and offers solutions for better laboratory practice and training.

Area of Science:

  • Pathology
  • Laboratory Medicine
  • Histotechnology

Background:

  • Immunohistochemistry (IHC) is integral to surgical pathology.
  • The rapid expansion of IHC presents challenges in standardization and training.
  • Lack of standardized training contributes to diagnostic pitfalls.

Purpose of the Study:

  • To define common pitfalls in diagnostic IHC.
  • To provide solutions for improving IHC practices in surgical pathology.
  • To address challenges in antibody reagent selection, detection systems, and instrumentation.

Main Methods:

  • Review of common diagnostic immunohistochemistry errors.
  • Analysis of factors contributing to IHC pitfalls.
  • Literature review on best practices in IHC.

Related Experiment Videos

Main Results:

  • Frequent pitfalls identified in diagnostic IHC.
  • Challenges in optimizing antibody reagents, detection systems, and instrumentation.
  • Inadequate incorporation of IHC into pathology training programs.

Conclusions:

  • Diagnostic IHC pitfalls are increasing.
  • Standardization of IHC protocols and training is essential.
  • Addressing these pitfalls will improve diagnostic accuracy in surgical pathology.