A numerical procedure for choosing effective, low toxicity plasticizers for glycol methacrylate embedding

A numerical method for selecting low toxicity plasticizers for glycol methacrylate (GMA) embedding mixtures is described. It involves use of various numerical parameters, namely; molecular weight, melting point, solubility parameter, and toxicity data. On the basis of these parameters, nine plasticizers were selected. Their effects on microtomy and tissue processing, and also their influence on tissue morphology and staining, were investigated. For tissue processed at low temperatures into GMA, using ethanol dehydration, the following compounds were as satisfactory as 2-butoxyethanol whilst being less toxic: 2-isopropoxyethanol, 2-(2-methoxyethoxy)ethanol, 2-(2-ethoxyethoxy)ethanol, 2-(n-butoxyethoxy)ethanol. However for tissues processed using the plasticizer as dehydrating agent, the optimum plasticizers were 2-isopropoxyethanol, 2-(2-methoxyethoxy)ethanol and 2-(2-ethoxyethoxy)ethanol. It is possible to give a numerical specification of the preferred plasticizers, and for the first procedure this is: a solubility parameter in the range 21-26 J0.5 cm-1.5 or higher, a melting point well below 0 degree C, and a rat oral LD50 greater than 12.52 mmol kg-1. It was also possible to analyse the differential effects of the dehydrating agents on histochemical and enzyme histochemical staining on a numerical basis, using a structure-activity relations approach.