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Updated: Jul 19, 2026
Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells
Published on: May 31, 2024
[Effect of Yishenqinghuo compound on biological characteristics of rat bone marrow stromal cells]
1Dept. of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, Shanghai 200011, China.
Objective:
To evaluate the effect of Yishenqinghuo recipe on biological characteristic of rat bone marrow stromal cells (BMSCs) and search for the function and mechanism of this recipe in treatment of periodontitis.
Methods:
12- to 15-month-old SD rats were allocated into 5 groups. Group A: control group (with no periodontitis model, fed with the same dosage of saline as Group D); Group B: model group (with periodontitis model, fed with the same dosage of saline as Group D); Group C: high dosage group (with periodontitis model, fed with a high dosage of medicine); Group D: middle dosage group (with periodontitis model, fed with a middle dosage of medicine); and Group E: low dosage group (with periodontitis model, fed with a low dosage of medicine). Ten days later, serums were collected from all the five groups for in vitro cultivation of BMSCs.
Results:
There was displayed a similarity in effect between the serum collected from Group C1 (serum collected 0.5 h after the last gavage of Group C) and that from Group A after the last gavage, the effect being the best in terms of proliferation of BMSCs. A comparison with the other groups had revealed a striking difference (P < 0.01), with Group B having turned out to be the worst. The serum from Group C2 (serum collected 1 h after the last gavage of Group C) after the last gavage could best enhance the generation and activity of ALP, having demonstrated a significant difference (P < 0.01) in comparison with the other groups.
Conclusion:
Yishenqinghuo recipe can improve the proliferation of BMSCs and facilitate the differentiation to osteoblast.
