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Published on: April 15, 2021
[Tension-force induced cyclooxygenase-2 expression mediated by microfilament in human periodontal ligament
Pei-Ying Xiong1, Sheng-Gao Huang, Jian-Xing Zhang
1Dept. of Stomatology, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To study the role of microfilament polymerization in menchanotransduction by human periodontal ligament fibroblast (hPDLFs).
Methods:
In tension-force group, hPDLFs were treated by tension-force values of 18% for 8 h, 16 h, 24 h. In tension-force and inhibitor group, the sample was treated with 5 microg/mL cytochalasin B before using tension-forece. Each sample was collected and the expression of cyclooxygenase-2 was measured by using immunohistoche staining.
Results:
In tension-force group, the expression of cyclooxygenase-2 enhanced with the extension of loading time. In tension-force and inhibitor group, cyclooxygenase-2 expression was depressed and had no relation with loading time.
Conclusion:
Tension-force induced cyclooxygenase-2 expression is mediated by microfilament, disruption of the microfilament polymerization will destroy mechanotransduction in hPDLFs.
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