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Related Experiment Video

Updated: Jul 19, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Characterization of differential gene expression profiles in diabetic embryopathy using DNA microarray analysis.

E Albert Reece1, Ilwoon Ji, Ying-King Wu

  • 1Department of Obstetrics and Gynecology, The Arkansas Center for Birth defects Research and Prevention, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

American Journal of Obstetrics and Gynecology
|September 27, 2006
PubMed
Summary

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Maternal diabetes alters gene expression in developing embryos, impacting key pathways. This study reveals changes in yolk sac cells, highlighting new insights into diabetic embryopathy.

Area of Science:

  • Developmental Biology
  • Genomics
  • Reproductive Medicine

Background:

  • Maternal diabetes is a known risk factor for congenital abnormalities.
  • The precise molecular mechanisms underlying diabetic embryopathy remain largely unknown.
  • Understanding these mechanisms is crucial for preventing birth defects in diabetic pregnancies.

Purpose of the Study:

  • To identify specific developmental genes and molecular pathways affected by maternal diabetes during embryogenesis.
  • To compare gene expression profiles in yolk sacs of diabetic and control rat embryos.
  • To elucidate the molecular basis of diabetic embryopathy.

Main Methods:

  • Induction of diabetes in rats using streptozotocin, with controlled and uncontrolled hyperglycemia groups.

Related Experiment Videos

Last Updated: Jul 19, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

  • Collection of yolk sacs from embryos at gestational day 12.
  • DNA microarray analysis of approximately 10,000 genes to compare expression profiles.
  • Validation of key gene expression changes using real-time polymerase chain reaction (PCR).

    • Main Results:

    • A total of 101 genes exhibited differential expression between diabetic and control embryo yolk sacs.
    • Identified affected pathways include insulin signaling, stress response, cell growth, calcium signaling, and protein kinase C (PKC) signaling.
    • Specific genes involved in these pathways were pinpointed, such as insulin 2, GAP43, and PKCBP beta15.

    Conclusions:

    • Diabetic embryopathy is associated with significant alterations in the expression of developmental and stress-response genes.
    • Yolk sac cells express insulin during early embryonic development, a novel finding.
    • Hyperglycemia in diabetic pregnancies leads to altered gene expression, causing aberrant cell signaling, morphogenesis, and ultimately, embryopathy.