Relationship between PTEN gene expression and differentiation of human glioma
1Department of Neurosurgery, Third Affiliated Hospital of Suzhou University, Changzhou, China.
Objective:
To investigate the relationship between PTEN gene expression and differentiation of glioma.
Material And Methods:
The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) method was applied to detect PTEN mRNA levels in glioma tissues. Tumor-adjacent normal tissues and benign brain tumors were used as controls. Relative PTEN mRNA levels were determined as the ratio of PTEN and GAPDH, which were correlated with the clinical-pathological results.
Results:
PTEN mRNA levels were significantly lower in the glioma tissues than in the benign brain tumors and tumor-adjacent normal tissues, whereas there were no statistical differences between benign brain tumor and the tumor-adjacent normal tissues. According to the pathological examinations, PTEN mRNA levels were higher in the high differential glioma than the low differential glioma.
Conclusions:
PTEN gene expression was suppressed in the glioma, which is related to the clinical-pathological results. It is suggested that determination of PTEN mRNA levels by RT-PCR could be a novel marker of disease classification.
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