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Related Concept Videos

Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
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Updated: Jun 30, 2026

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
11:20

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Published on: October 16, 2014

Eukaryotic expression: developments for structural proteomics.

A R Aricescu1, R Assenberg, R M Bill

  • 1Division of Structural Biology and Oxford Protein Production Facility, Wellcome Trust Centre for Human Genetics, Oxford, England.

Acta Crystallographica. Section D, Biological Crystallography
|September 27, 2006
PubMed
Summary
This summary is machine-generated.

High-throughput eukaryotic protein production methods were developed for structure determination when prokaryotic systems fail. These methods utilize yeast, insect, and mammalian cells for efficient protein expression and screening.

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Area of Science:

  • Structural biology
  • Molecular biology
  • Biochemistry

Background:

  • Prokaryotic expression systems are often insufficient for producing adequate viral and human proteins for structure determination.
  • Eukaryotic expression systems offer potential alternatives but require optimized high-throughput methodologies.

Purpose of the Study:

  • To develop and implement high-throughput (HTP) methodologies for protein production in eukaryotic systems.
  • To assess the efficacy of yeast, baculovirus-infected insect cells, and mammalian cells for HTP protein production.
  • To evaluate strategies for cloning, expression screening, and protein production pipelines.

Main Methods:

  • Development and implementation of HTP cloning vectors.
  • Expression screening across three eukaryotic systems: yeast (Pichia pastoris, Saccharomyces cerevisiae), baculovirus-infected insect cells, and transient mammalian cell expression.
  • Assessment of co-expression, selenomethionine labeling, and glycosylation control strategies.

Main Results:

  • Successful application of HTP methodologies in eukaryotic systems for protein production.
  • Characterization of suitable vectors for HTP cloning and expression screening.
  • Evaluation of strategies for enhancing protein production, labeling, and post-translational modifications.

Conclusions:

  • Eukaryotic expression systems, when coupled with HTP methodologies, can overcome limitations of prokaryotic systems for protein production.
  • The developed strategies facilitate efficient cloning, screening, and production of proteins for structural studies.
  • These advancements are crucial for advancing structural proteomics and understanding viral and human protein functions.