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Determination of Crystal Structures

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...

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Related Experiment Video

Updated: Jul 19, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance

Ian M Berry¹, O Dym, R M Esnouf

¹Division of Structural Biology and Oxford Protein Production Facility (OPPF), Wellcome Trust Centre for Human Genetics, Oxford, England.

Acta Crystallographica. Section D, Biological Crystallography

|September 27, 2006

Summary

High-throughput and nanolitre-scale protein crystallography technologies have been successfully adopted by Structural Proteomics In Europe (SPINE) partners. This revolutionizes structural biology methods and sets the stage for future crystallization advancements.

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Last Updated: Jul 19, 2026

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Published on: February 28, 2019

Area of Science:

Structural biology
Biochemistry
Biophysics

Background:

The Structural Proteomics In Europe (SPINE) project commenced in October 2002.
Workpackage 4 focused on advancing protein crystallography technologies.

Purpose of the Study:

To review developments in high-throughput and nanolitre-scale protein crystallography.
To assess the adoption and impact of new technologies within the SPINE project.

Main Methods:

Surveying the uptake and use of novel technologies by SPINE partners across Europe.
Analyzing partner experiences with new working methods.

Main Results:

Highly successful adoption of innovative protein crystallography techniques was observed.
New methods are revolutionizing the field of structural biology.

Conclusions:

The implementation of advanced protein crystallography technologies has been effective.
Future developments in crystallization methodologies are anticipated.