Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

NMR in the SPINE Structural Proteomics project.

E Ab1, A R Atkinson, L Banci

  • 1Bijvoet Center for Biomolecular Research, NMR Spectroscopy, Utrecht University, Padualaan 8, CH Utrecht, The Netherlands.

Acta Crystallographica. Section D, Biological Crystallography
|September 27, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles
This summary is machine-generated.

The Structural Proteomics In Europe (SPINE) consortium enhanced nuclear magnetic resonance (NMR) spectroscopy pipelines for high-throughput protein structure determination. This improved success rates, delivering over 60 protein structures, including those resistant to crystallization.

Area of Science:

  • Structural biology
  • Biophysics
  • Proteomics

Background:

  • The Structural Proteomics In Europe (SPINE) consortium aimed to advance protein structure determination.
  • Nuclear Magnetic Resonance (NMR) spectroscopy is a key technique in structural biology.
  • High-throughput (HTP) methods are crucial for large-scale proteomics studies.

Purpose of the Study:

  • To describe the developments and contributions of NMR spectroscopy groups within the SPINE consortium.
  • To highlight advancements in HTP NMR pipelines for protein structure determination.
  • To assess the impact of integrated NMR methods on overall structure determination success rates.

Main Methods:

  • Development of HTP pipelines covering sample preparation, data acquisition, processing, and analysis for NMR.

Related Experiment Videos

  • Implementation of protonless (13)C-direct detection methods.
  • Application of inferential structure determination (ISD) techniques.

    • Main Results:

    • Significant improvements in sensitivity, automation, speed, robustness, and validation of NMR structure determination processes.
    • Successful determination of over 60 protein structures using HTP NMR pipelines.
    • Determination of five protein structures that were previously unachievable via crystallization.

    Conclusions:

    • The integration of advanced NMR spectroscopy into structural proteomics pipelines significantly enhances protein structure determination.
    • HTP NMR methods, including novel detection and determination strategies, are vital for overcoming challenges in structural biology.
    • SPINE consortium's efforts have demonstrably improved the efficiency and scope of protein structure determination.