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Related Experiment Video

Updated: Jul 19, 2026

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

GRETA, a new multifermenter system for structural genomics and process optimization.

Marie Hedrén¹, Andras Ballagi, Lars Mörtsell

¹Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.

Acta Crystallographica. Section D, Biological Crystallography

|September 27, 2006

Summary

The GRETA automated multifermenter system enhances protein production for structural genomics. This system yields significantly more protein per culture volume with minimal manual input compared to traditional methods.

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Area of Science:

Biotechnology
Structural Biology
Proteomics

Background:

Structural genomics projects require efficient protein production.
Current methods like baffled-flask protocols have limitations in yield and scalability.
The Structural Proteomics In Europe (SPINE) project aimed to improve these processes.

Purpose of the Study:

To develop and optimize the GRETA automated multifermenter system for structural genomics.
To compare protein production yields between GRETA and traditional baffled-flask methods.
To assess the utility of GRETA for scale-up and fermentation process optimization.

Main Methods:

The GRETA system, featuring 6-24 parallel fermentation chambers with individual parameter control (temperature, stirring, pH, dissolved oxygen, feed profiles), was utilized.
Six human proteins were used to optimize GRETA fermentation processes.
GRETA processes were compared against standard baffled-flask protocols.

Main Results:

Optimized GRETA processes achieved several times higher protein yield per litre of culture compared to baffled-flask protocols.
GRETA required limited manual intervention during fermentation.
The system demonstrated efficient and reproducible protein production.

Conclusions:

The GRETA automated multifermenter system is a valuable tool for structural proteomics.
GRETA offers a significant improvement in protein yield and efficiency for structural genomics.
It serves as a viable alternative for scale-up production and fermentation process optimization.