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[Strategies for antibody immobilization during the fabrication of antibody microarrays]

Lu Zhang1, Bicheng Liu

  • 1Institute of Nephrology, Zhongda Hospital, Southeast University, Nanjing 210009, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
|September 28, 2006
PubMed

As one of the proteomic technologies, antibody microarrays can provide a powerful tool for the high-throughput parallel analysis of thousands of proteins in a single experiment. Because antibodies are chemically and structurally complex; immobilizing antibody on solid phase surface of substrates has been a key step during the development of antibody microarrays. This review will focus on the current strategies for antibody immobilization and discuss the advantages and disadvantages of these methods.

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