A neurofibrillar staining method for retina and skin: a simple modification for improved staining and reliability

A simple modification of a reduced silver-stain for neurofilaments is described. Using neutral pH, perfusion fixation, and heat treatment during postfixation this method greatly improves the quality and reliability of staining of ganglion cells in retinal wholemounts from primate and other species. In addition it permits the selective staining of some cell populations thought to be refractory to silver stains. The method also stains cutaneous receptors in thick sections of the glabrous skin.