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Updated: Jul 12, 2026
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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
Monitoring DPA release from a single germinating Bacillus subtilis endospore via surface-enhanced Raman scattering
David D Evanoff1, John Heckel, Thomas P Caldwell
1Department of Chemistry, Clemson University, Clemson, SC 29634, USA.
Journal of the American Chemical Society
|September 28, 2006
Abstract:
A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.
