Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A strategy for annotating the human milk glycome.

Milady R Ninonuevo1, Youmie Park, Hongfeng Yin

  • 1Department of Chemistry and Biochemistry (School of Medicine), University of California, Davis, California 95616, USA.

Journal of Agricultural and Food Chemistry
|September 28, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Digital Revitalization of a Legacy Linear Ion Trap System.

Analytical chemistry·2026
Same author

A multi-glycomic tool for characterization of resistant starch.

Carbohydrate polymers·2026
Same author

LOESS-based normalization workflow for targeted HDL glycoproteomics in an Alzheimer's disease cohort.

RSC advances·2026
Same author

Selective Human-Milk-Inspired Antimicrobial Peptides for the Treatment of Bacterial Vaginosis.

Pharmaceutics·2026
Same author

Neonatal Enteric Infection Disrupts the Microbiota-Gut-Brain Axis Through Pattern Recognition Receptors and Altered Neuroimmune Signaling.

bioRxiv : the preprint server for biology·2026
Same author

Accessing Higher Order Mathieu Space Stability Zones to Narrow Isolation Widths Using Digital Quadrupole Time-of-Flight Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2025

Human milk oligosaccharides, though not nutritive, are vital for infant gut microflora and immune development. New mass spectrometry methods precisely identify their diverse structures and variations between individuals.

Area of Science:

  • Biochemistry
  • Immunology
  • Microbiology

Background:

  • Human milk oligosaccharides (HMOs) are abundant, diverse, bioactive molecules crucial for infant development.
  • HMOs are hypothesized to influence the intestinal microflora and mucosal immune system development.
  • Accurate determination of HMO composition is essential for understanding their biological functions.

Purpose of the Study:

  • To develop and apply advanced mass spectrometry techniques for detailed analysis of HMO composition.
  • To investigate variations in HMO profiles among different human milk samples.
  • To establish reliable methods for identifying and differentiating HMO structures.

Main Methods:

  • Analysis of HMOs using a combination of HPLC-Chip/MS and MALDI-FT ICR MS.

Related Experiment Videos

  • Accurate mass measurements via orthogonal time-of-flight (o-TOF) mass spectrometry.
  • Tandem MS coupled with exoglycosidase digestion for structural isomer differentiation.

    • Main Results:

    • Identification of approximately 200 individual HMO molecular species through accurate mass measurements.
    • HPLC-Chip/MS profiling revealed significant variations in HMO composition among samples from five different women.
    • Established HPLC-Chip/MS as a routine method for HMO identification.

    Conclusions:

    • Advanced mass spectrometry techniques enable comprehensive analysis of complex HMO mixtures.
    • Significant inter-individual variability exists in human milk oligosaccharide composition.
    • Tandem MS with exoglycosidase digestion offers precise structural elucidation of HMO isomers, advancing research into their biological roles.