Hole-enhanced Raman scattering
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Updated: Jul 19, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
Published on: July 25, 2022
John T Bahns1, Funing Yan, Dengli Qiu
1Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439, USA.
Abstract:
Stokes and anti-Stokes non-resonant hole-enhanced Raman scattering (HERS) spectra with high signal-to-noise ratio (S/N) are reported for the first time for aqueous phase R6G molecules adsorbed onto random nanoholes in thin gold films. Compared to conventional surface-enhanced Raman scattering from nanometric gold colloid particles, HERS exhibits higher strength gain, exceptional reproducibility, simple and reliable substrate preparation, and excellent mechanical stability. By correlating the hole density with Raman scattering gain, we determined optimum HERS gain for 50 nm diameter holes at approximately 100 holes/microm(2). Providing a Raman substrate with uniform "hot spots", we expect that HERS will make the quantitative Raman analysis of biological molecules possible.
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