Related Experiment Video
Updated: Jul 19, 2026

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
Published on: November 11, 2016
Sequence variability of retroviral particles derived from human melanoma cells melanoma-associated retrovirus
Sonja Hirschl1, Oliver Schanab, Heide Seppele
1Department of Dermatology, Division of General Dermatology, University of Vienna Medical School, Währinger Gürtel 18-20, Vienna, Austria. sonja.hirschl@mci.edu
Abstract:
We have shown that melanoma cells produce viral particles that contain sequences which are homologous to human endogenous retroviruses. In this study particles derived from different melanoma cell lines and from melanoma cells of a lymph node metastasis were characterized. We determined the density and the reverse transcriptase (RT) activity of viral particles. Furthermore, we analyzed the sequence variability of multiple clones of each particle preparation. The particles were found to package sequences, which vary for each of the analyzed cell lines. Moreover, even particles derived from the same cell line contain heterologous sequences.
Related Concept Videos
Retroviruses
Mechanisms of Retrovirus-induced Cancers
Mechanisms of Retrovirus-induced Cancers
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
Retrovirus Life Cycles
Non-LTR Retrotransposons

