Hemogram changes in lactating dairy cows given human recombinant granulocyte colony stimulating factor...

Area of Science:

• Veterinary Science
• Hematology
• Immunology

Background:

• Mammary gland infections are a concern in dairy cattle.
• Colony-stimulating factors (CSFs) can modulate immune cell production.
• Understanding bovine hematologic responses to CSFs is crucial for therapeutic development.

Purpose of the Study:

• To evaluate hematologic changes in lactating dairy cattle after administration of human recombinant colony stimulating factor (Hr-GCSF).
• To determine if Hr-GCSF induces dose- and time-dependent hematologic alterations.
• To explore potential therapeutic applications of Hr-GCSF in bovine health.

Main Methods:

• Lactating dairy cows were administered two dosage regimes of Hr-GCSF (1 µg/kg and 3 µg/kg).
• Hematologic parameters, including white blood cell and neutrophil counts, were monitored over time.
• Control groups received no Hr-GCSF administration.
• Milk somatic cell counts were assessed.

Main Results:

• A screening dose (1 µg/kg) of Hr-GCSF increased mature neutrophil counts by 3-4 fold by day 12 (P < 0.043).
• A priming dose (3 µg/kg) of Hr-GCSF increased mature neutrophil counts by 3-5 fold and white blood cell counts by 2-3 fold by day 5 (P < 0.05).
• No significant changes in neutrophil or milk somatic cell counts were observed in the control group.

Conclusions:

• Hr-GCSF administration induces significant, dose- and time-dependent increases in neutrophil counts in lactating dairy cattle.
• Hr-GCSF shows potential as a therapeutic agent to enhance immune responses in cattle.
• Further research into CSF applications for infectious diseases in livestock is warranted.