Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biodiversity buffers forest ecosystems from compound climate extremes.

Nature communications·2026
Same author

Prognostic incremental value of perivascular adipose tissue in myocardial infarction with non-obstructive coronary arteries: a multicenter cohort study.

Quantitative imaging in medicine and surgery·2026
Same author

Scaling biodiversity-stability relationships from populations to meta-communities across trophic levels.

Nature communications·2026
Same author

Divergence From Temperate Models: Pollution Dominance and Stochastic Assembly in a Continental Freshwater System Under Compressed Modernity.

Global change biology·2026
Same author

Global synthesis reveals systematic variation in trophic transfer efficiency across and within ecosystems.

Science advances·2026
Same author

Statistical shape refinement and genetic algorithm calibration of design response spectra based on strong-motion records.

PloS one·2026

Related Experiment Video

Updated: Jul 19, 2026

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
10:18

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Published on: August 27, 2016

3D inverted opal hydrogel scaffolds with oxygen sensing capability.

Yuanfang Liu1, Shaopeng Wang

  • 11024S Innovation Way, Stillwater, OK 74074, USA.

Colloids and Surfaces. B, Biointerfaces
|September 29, 2006
PubMed
Summary

Researchers developed a novel 3D cell scaffold for measuring local oxygen levels in 3D cell cultures. This luminescence-based oxygen sensing scaffold allows for 3D mapping of oxygen during cell growth, addressing a key challenge in cell culture monitoring.

More Related Videos

Self-reporting Scaffolds for 3-Dimensional Cell Culture
14:49

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs
07:48

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Published on: May 5, 2023

Related Experiment Videos

Last Updated: Jul 19, 2026

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
10:18

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Published on: August 27, 2016

Self-reporting Scaffolds for 3-Dimensional Cell Culture
14:49

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs
07:48

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Published on: May 5, 2023

Area of Science:

  • Biomaterials Engineering
  • Cell Biology
  • Biomedical Optics

Background:

  • Measuring local oxygen levels in 3D cell cultures is crucial for understanding cellular processes but presents significant challenges.
  • Existing methods often lack the spatial resolution or compatibility with 3D environments.

Purpose of the Study:

  • To develop a novel 3D cell scaffold with integrated luminescence-based oxygen sensing capabilities.
  • To enable 3D mapping of oxygen distribution within cell cultures.

Main Methods:

  • Fabrication of a hydrogel inverted opal scaffold using poly(2-hydroxyethyl methacrylate) (pHEMA) and poly(methacryloyloxy)ethyl-trimethylammonium chloride) (pMEATAC) monomers.
  • Incorporation of Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium chloride (Ru(dpp)(3)) via layer-by-layer assembly for oxygen sensing.
  • Coating with a pHEMA-pMEATAC copolymer for protection and culturing of human bone marrow stromal cells (HS-5).

Main Results:

  • The developed scaffold demonstrated luminescence-based oxygen sensing, where Ru(dpp)(3) emission is dynamically quenched by oxygen.
  • Transparent scaffolds allowed for 3D fluorescence intensity mapping using confocal microscopy.
  • Successful culture of HS-5 cells on the scaffold showed decreased oxygen levels in cell-rich areas, correlating with cell consumption.

Conclusions:

  • The novel 3D scaffold effectively measures local oxygen levels in 3D cell cultures.
  • This technology facilitates 3D oxygen mapping, providing valuable insights into cellular microenvironments.
  • The scaffold supports cell viability and function, opening avenues for advanced cell culture monitoring.