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MultiCode-RTx real-time PCR system for detection of subpopulations of K65R human immunodeficiency virus type 1

Evguenia S Svarovskaia1, Michael J Moser, Andrew S Bae

  • 1Gilead Sciences, Inc., 4 University Place, 4611 University Drive, Durham, NC 27707, USA. jenny.svarovskaia@gilead.com

Journal of Clinical Microbiology
|September 29, 2006
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...