Related Experiment Video
Updated: Jul 19, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
Published on: November 12, 2012
Functional analysis of 11 putative essential genes in Bacillus subtilis
Alison Hunt1, Joy P Rawlins1, Helena B Thomaides2
1Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.
Abstract:
Systematic inactivation of Bacillus subtilis genes has previously revealed that 271 are indispensable for growth. In the present study, 11 of these (yacA, ydiB, ydiC, ykqC, ylaN, yloQ, ymdA, yneS, yqeI, yqjK and ywlC) were identified as genes encoding proteins of unknown function. By analysing the effects of protein depletion, and examining the subcellular localization of these proteins, a start has been made in elucidating their functions. It was found that four of these genes (ydiB, yloQ, yqeI and ywlC) were not required for B. subtilis viability. Analysis of the localization of YkqC suggests that it co-localizes with ribosomes, and it is proposed that it is involved in processing either rRNA or specific mRNAs when they are associated with the ribosome. The results suggest that other novel essential proteins may be involved in lipid synthesis and control of cell wall synthesis.
More Related Videos
13:28Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
09:27Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
Published on: June 24, 2016
Related Concept Videos
Coordination of Gene Expression Processes in Bacteria
Modern Molecular Taxonomy
Gene Regulation During Sporulation
Regulation of Bacterial Virulence
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Applications of Molecular Taxonomy