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Related Experiment Videos

Xenopus oocyte K+ current. III. Phorbol esters and pH regulate current at gap junctions.

L J Greenfield1, J T Hackett, J Linden

  • 1Department of Physiology, University of Virginia Health Sciences Center, Charlottesville 22908.

The American Journal of Physiology
|November 11, 1990
PubMed
Summary

Adenosine 3

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Area of Science:

  • Cellular biology
  • Reproductive biology
  • Ion channel physiology

Background:

  • Xenopus oocytes are surrounded by follicle cells connected via gap junctions.
  • Adenosine 3',5'-cyclic monophosphate (cAMP) stimulates an outward K+ current in these follicles.
  • Follicle cell removal or phorbol ester treatment abolishes this cAMP-stimulated current.

Purpose of the Study:

  • To investigate the role of gap junctions in mediating cAMP-stimulated K+ currents in Xenopus follicles.
  • To determine the effects of phorbol esters and acidic pH on gap junctional communication and K+ currents.
  • To elucidate the mechanism by which cAMP influences K+ currents in oocyte-follicle cell complexes.

Main Methods:

  • Voltage clamp electrophysiology to measure K+ currents and electrical coupling.
  • Treatment with phorbol esters and acidic pH to modulate gap junctions.
  • Assessment of cAMP levels in follicles.
  • Computer simulations to analyze electrical coupling effects.

Main Results:

  • Phorbol esters and acidic pH inhibit the cAMP-stimulated K+ current without reducing cAMP levels.
  • These agents decrease electrical coupling between oocytes by reducing gap junctional conductance.
  • Increased cAMP enhances gap junctional conductance and K+ currents.
  • Simulations confirm that reduced electrical coupling is not due to K+ channel blockade.

Conclusions:

  • cAMP-activated K+ currents in Xenopus follicles are modulated by gap junctional communication.
  • Phorbol esters and acidic pH reduce K+ currents by decreasing gap junctional conductance.
  • The findings suggest that cAMP-activated K+ currents originate in follicle cells and are transmitted to the oocyte via gap junctions.

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