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Updated: Jul 19, 2026
08:12
Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets
Published on: September 6, 2019
Coxsackievirus B3 in human milk
Mei-Ling Chang1, Kuo-Chien Tsao, Chi-Chia Huang
1Division of Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Tiawan.
The Pediatric Infectious Disease Journal
|September 29, 2006
Summary
Coxsackievirus B3 was detected in breast milk of mothers with severely infected neonates using RT-PCR. The virus was undetectable in milk 12-14 days after symptom onset, indicating a limited transmission window.
Area of Science:
- Virology
- Neonatal Medicine
- Infectious Diseases
Background:
- Neonatal disease caused by Coxsackievirus B3 (CVB3) presents a significant mortality risk.
- Understanding transmission routes is crucial for preventing severe outcomes in newborns.
Observation:
- This study reports the first detection of CVB3 in maternal breast milk from two cases of severe neonatal infection.
- Both culture and reverse transcriptase-polymerase chain reaction (RT-PCR) methods were employed for virus identification.
Findings:
- RT-PCR demonstrated rapid and sensitive detection of CVB3 in breast milk.
- CVB3 was no longer detectable in the breast milk of symptomatic mothers 12 to 14 days post-symptom onset.
Implications:
- RT-PCR is a valuable tool for rapid diagnosis of CVB3 in breast milk.
- The findings suggest a potential, time-limited window for CVB3 transmission via breastfeeding.
- Further research is needed to confirm the clinical significance of CVB3 in breast milk for neonatal health.
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