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Armored RNA as positive control and standard for quantitative reverse transcription-polymerase chain reaction assay

L Zhao1, Y Ma, S Zhao

  • 1Department of Laboratory, Tai'an Central Hospital, Tai'an, China [corrected]

Archives of Virology
|September 29, 2006
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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