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A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus
K Muraiso1, M Hijikata, S Ohkoshi
1Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochemical and Biophysical Research Communications
|October 30, 1990
Abstract:
A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.