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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
p53 tumour suppressor gene polymorphism is associated with recurrent implantation failure
C Kay1, R S Jeyendran, C B Coulam
1Andrology Laboratory Services, Chicago, IL, USA.
Reproductive Biomedicine Online
|September 30, 2006
Summary
The p53 codon 72 proline variant (Pro72) is more common in women with recurrent implantation failure after IVF. This genetic factor may increase susceptibility to implantation failure, impacting pregnancy success.
Area of Science:
- Reproductive Medicine
- Genetics
- Oncology (Tumor Suppressor Genes)
Background:
- Recurrent implantation failure is a primary obstacle to achieving pregnancy following in vitro fertilization (IVF).
- Successful embryo implantation necessitates trophoblast invasion and the development of a maternal blood supply (angiogenesis).
- The p53 tumor suppressor gene plays a critical role in regulating cell growth and promoting angiogenesis.
Purpose of the Study:
- To investigate the association between p53 codon 72 polymorphism and recurrent implantation failure (RIF).
- To determine if specific p53 variants act as a genetic risk factor for implantation failure after IVF and embryo transfer.
Main Methods:
- DNA was extracted from buccal swabs of 70 women with a history of implantation failure.
- Polymerase chain reaction (PCR) was used to amplify and identify p53 arginine (Arg72) and proline (Pro72) variants.
- Genotype frequencies were compared between women with RIF, women with recurrent pregnancy loss (n=205), and controls (n=20).
Main Results:
- The frequency of the p53 Pro72 variant was significantly higher in women experiencing recurrent implantation failure compared to those with recurrent miscarriage and control groups (P=0.003).
- Analysis included comparisons of homozygous Arg/Arg, Pro/Pro, and heterozygous Arg/Pro genotypes.
Conclusions:
- p53 codon 72 polymorphism, particularly the Pro72 variant, may represent a susceptibility factor for recurrent implantation failure.
- This genetic predisposition could influence the success rates of IVF and embryo transfer procedures.
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