Repository of human embryonic stem cell lines and development of individual specific lines using stembrid technology

A human embryonic stem cell (HESC) line repository has been established, containing HESC lines with normal and abnormal genotypes, providing the source for studying the primary mechanisms of genetic disorders at the cellular level. Because the outcome of HESC transplantation treatment depends on access to human leukocyte antigen identical stem cells, the development of individual specific HESC was initiated, using the original stembrid technology, which is based on the hybridization of adult somatic cells with cytoplast of HESC lines. The data presented here demonstrate feasibility of this approach in the future development of HESC transplantation treatment of genetic and acquired disorders. The established HESC repository presently contains 166 HESC lines, including 127 with normal genotype and 39 with genetic and chromosomal disorders.