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Updated: Jul 19, 2026

Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
Peroxisome targeting signal 1: is it really a simple tripeptide?
Cécile Brocard1, Andreas Hartig
1Max F Perutz Laboratories, University of Vienna, Department of Biochemistry, Dr. Bohrgasse 9, 1030 Vienna, Austria. Cecile.Brocard@univie.ac.at
Abstract:
Originally, the peroxisomal targeting signal 1 (PTS1) was defined as a tripeptide at the C-terminus of proteins prone to be imported into the peroxisomal matrix. The corresponding receptor PEX5 initiates the translocation of proteins by identifying potential substrates via their C-termini and trapping PTS1s through remodeling of its TPR domain. Thorough studies on the interaction between PEX5 and PTS1 as well as sequence-analytic tools revealed the influence of amino acid residues further upstream of the ultimate tripeptide. Altogether, PTS1s should be defined as dodecamer sequences at the C-terminal ends of proteins. These sequences accommodate physical contacts with both the surface and the binding cavity of PEX5 and ensure accessibility of the extreme C-terminus. Knowledge-based approaches in applied Bioinformatics provide reliable tools to accurately predict the peroxisomal location of proteins not yet determined experimentally.
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