Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Micro hole-based cell chip with impedance spectroscopy.

Sungbo Cho1, Hagen Thielecke

  • 1Biohybrid Systems, Fraunhofer Institute for Biomedical Engineering, Ensheimer Str. 48, 66386 St. Ingbert, Germany. sungbo.cho@ibmt.fhg.de

Biosensors & Bioelectronics
|September 30, 2006
PubMed
Summary

This study shows micro hole chips effectively measure cell properties. Impedance changes reveal cell growth and membrane integrity without labeling, crucial for cell characterization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of zinc oxide levels and probiotic supplementation in weaning-to-finishing pig diets: productivity, gut microbial composition, and environmental implications.

Journal of animal science and technology·2026
Same author

Dopant-driven electronic and lattice softening enables transport-coupled CO<sub>2</sub> reduction in β-Ag<sub>2</sub>Se.

Physical chemistry chemical physics : PCCP·2026
Same author

Start-Up Circuits for Ultra-Low-Voltage Thermoelectric Energy Harvesting: A Topology-Oriented Review and Design Guide.

Nanomaterials (Basel, Switzerland)·2026
Same author

Structure-Guided Computational Design and Electrochemical Validation of a DNA Aptamer for Impedimetric Detection of Shiga-like Toxin 2.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

High stocking density triggers stress-induced physiological changes and alters nasal and fecal microbiota in finishing pigs.

Porcine health management·2026
Same author

Response of gut microbiome and metabolomic profiles to POLYCAN, a β-glucan derived from Aureobasidium pullulans SM-2001 in beagles.

Journal of animal science and biotechnology·2026

Area of Science:

  • Biophysics
  • Cellular Engineering
  • Electrical Engineering

Background:

  • Electric fields enable biological cell characterization and manipulation.
  • Micro hole-based chips offer an approach to mitigate electrode polarization effects.
  • Understanding the micro hole/cell interface electrical properties is vital for accurate cell analysis.

Purpose of the Study:

  • To investigate the electrical characteristics of a micro hole-based cell chip.
  • To determine the key factors influencing impedance measurements in micro hole cell chips.
  • To assess the potential of micro hole chips for monitoring single-cell behavior.

Main Methods:

  • Finite Element Method (FEM) simulations were employed to model electrical impedance.
  • Experimental impedance measurements were conducted on cells cultured on micro hole chips.

Related Experiment Videos

  • Cell adhesion/spread and intra-cellular space contributions to impedance were analyzed.

    • Main Results:

    • FEM simulations indicated that cell adhesion/spread significantly impacts impedance, more than intra-cellular space.
    • The effective frequency range for impedance related to cell state on the hole was below several kiloHertz.
    • Experimental results showed increased impedance with cultivation time and decreased impedance upon addition of culture medium with DMSO.

    Conclusions:

    • Micro hole-based chips are sensitive to cell adhesion and state.
    • The chip shows potential for label-free monitoring of single-cell growth and membrane integrity.
    • This technology could advance real-time cellular analysis and diagnostics.