Related Experiment Video
Updated: Jul 19, 2026

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
Published on: May 21, 2018
Synergism of CAY-1 with amphotericin B and itraconazole
A J De Lucca1, J M Bland, S Boue
1Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, New Orleans, La. 70124, USA. adelucca@srrc.ars.usda.gov
Background:
CAY-1 is a fungicidal saponin from cayenne pepper whose mode of action differs from amphotericin B (AB) and itraconazole (IT). This work determined CAY-1 synergism with AB or IT.
Methods:
CAY-1 was purified and used in checkerboard microdilution studies where CAY-1 and AB or IT were mixed with nongerminated (NG) and germinating (G) conidia of three Aspergillus species and Candida albicans. Inhibition was visually determined at 24 and 48 h.
Results:
CAY-1 had predominantly additive-synergistic interaction with AB or IT against the Aspergillus NG and G conidia. Excellent synergy between CAY-1 and AB occurred at 24 and 48 h against C. albicans. Results suggest CAY-1 enhances AB and IT efficacy.
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