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Advances in transgenic rat production.

Wanda E Filipiak1, Thomas L Saunders

  • 1Transgenic Animal Model Core, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Transgenic Research
|September 30, 2006
PubMed
Summary

Establishing a transgenic rat service requires reproducible methods. Researchers optimized superovulation, recipient preparation, and microinjection, finding Sprague-Dawley rat transgenesis more efficient than mouse models.

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Area of Science:

  • * Animal models
  • * Genetic engineering
  • * Reproductive biology

Background:

  • * Routine production of transgenic rats is crucial for research.
  • * Standardized protocols are needed for efficient generation of transgenic founders.
  • * Previous methods lacked consistency and optimization.

Purpose of the Study:

  • * To establish a reliable and reproducible method for transgenic rat production.
  • * To optimize key parameters affecting transgenesis efficiency in Sprague-Dawley and Fischer 344 rats.
  • * To compare the efficiency of transgenic rat production with that of mice.

Main Methods:

  • * Comparison of five superovulation regimens, selecting PMSG/HCG treatment.
  • * Evaluation of four methods for preparing pseudopregnant recipients, choosing estrus synchronization with LHRHa and mating.
  • * Optimization of microinjection technique using modified needles and DNA buffers, achieving >80% egg survival.

Main Results:

  • * Established protocols for producing transgenic founders in Sprague-Dawley (SD) and Fischer 344 (F344) rats.
  • * Identified optimal superovulation (20 IU PMSG/30 IU HCG) and recipient preparation (estrus synchronization) methods.
  • * Achieved high egg survival (>80%) post-microinjection.
  • * Demonstrated higher efficiency in SD rat transgenesis compared to C57BL/6J mice, requiring fewer donors and recipients per founder.

Conclusions:

  • * Optimized protocols enable predictable and reproducible transgenic rat production.
  • * Transgenic Sprague-Dawley rat production is more efficient than in C57BL/6J mice.
  • * These advancements can increase the accessibility of rat models for research.

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