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Epinephrine-binding plasma-membrane antigens in rat liver
European Journal of Biochemistry
|August 15, 1975
Summary
Rat liver plasma membranes contain multiple enzyme complexes that bind epinephrine. These multienzyme complexes exhibit nucleoside di- and triphosphatase, NADH-neotetrazolium reductase, and L-leucyl-beta-naphthylamidase activities, with some binding hormones.
Area of Science:
- Biochemistry
- Cell Biology
- Membrane Biology
Background:
- Rat liver plasma membranes contain various enzyme activities.
- Previous studies identified some membrane antigens with enzymatic functions.
Purpose of the Study:
- To characterize enzyme activities and hormone-binding properties of rat liver plasma membrane antigens.
- To investigate the association of enzymatic activities with hormone-binding components.
Main Methods:
- Two-dimensional immunoelectrophoresis of detergent extracts from rat liver plasma membranes.
- Enzyme staining and autoradiography to detect specific activities and hormone binding.
- Sepharose 4B chromatography to analyze molecular weights of antigens.
Main Results:
- Identified approximately ten antigens with nucleoside di- and triphosphatase activity, many also showing NADH-neotetrazolium reductase activity.
- Two antigens displayed L-leucyl-beta-naphthylamidase activity and bound [14C]epinephrine.
- High molecular weight nucleoside di- and triphosphatases (5,000,000) were associated with L-leucyl-beta-naphthylamidase activity.
Conclusions:
- Rat liver plasma membranes possess multienzyme complexes capable of binding epinephrine.
- Enzymatic activities like nucleoside di- and triphosphatase and L-leucyl-beta-naphthylamidase are associated with hormone-binding membrane components.
- These hormone-binding membrane components exist in various molecular weight fractions.