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The expression of the neonatal sarcoplasmic reticulum Ca2+ pump (SERCA1b) hints to a role in muscle growth and

Erno Zádor1, Peter Vangheluwe, Frank Wuytack

  • 1Institute of Biochemistry, Faculty of Medicine, University of Szeged, H-6720 Szeged, Dóm tér 9, P.O. Box 427, Hungary. erno@biochem.szote.u-szeged.hu

Cell Calcium
|October 3, 2006
PubMed

Insights

The neonatal sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 1 (SERCA1b) protein is found in developing muscle cells but not adult muscles. Its expression is regulated at the protein level, suggesting a role in muscle growth.

Area of Science:

  • Muscle physiology
  • Molecular biology
  • Biochemistry

Background:

  • The neonatal sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 1 (SERCA1b) isoform has known developmental regulation at the transcript level.
  • However, its protein expression and functional significance remain largely undefined.

Purpose of the Study:

  • To investigate the protein expression and cellular localization of SERCA1b.
  • To elucidate the role of SERCA1b in muscle development and regeneration.

Main Methods:

  • Generation of specific antibodies for SERCA1b detection.
  • Immunohistochemistry on cultured and regenerating muscle tissues.
  • Analysis of myogenic cell lines (C2C12, sol8, BC3H1) and mouse diaphragm tissue.

Main Results:

  • SERCA1b protein is exclusively expressed in myoblasts and myotubes of cultured and regenerating muscle.
  • SERCA1b protein is undetectable in normal adult fast and slow muscles.
  • SERCA1b is the primary SERCA1 isoform induced during differentiation in vitro, present in myotubes.
  • BC3H1 cells express SERCA1b mRNA but not protein, indicating post-transcriptional regulation.
  • SERCA1b protein is absent in denervated/stretched adult soleus despite mRNA upregulation.
  • SERCA1b constitutes most SERCA1 expression in newborn mouse diaphragm.

Conclusions:

  • SERCA1b expression is significantly regulated at the protein level, not just transcriptionally.
  • SERCA1b plays a crucial role in the development and growth of skeletal muscle.
  • The absence of SERCA1b may contribute to diaphragm dysfunction in SERCA1 null mutant mice.

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