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Related Experiment Videos

How do patch clamp seals form? A lipid bleb model.

R L Milton1, J H Caldwell

  • 1Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver, 80262.

Pflugers Archiv : European Journal of Physiology
|August 1, 1990
PubMed
Summary
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Single channel recordings using the patch voltage clamp method may not reflect native cell membranes. Ion channels in lipid blebs, not undisturbed membrane, might be responsible for observed biophysical properties.

Area of Science:

  • Biophysics
  • Cell Biology
  • Electrophysiology

Background:

  • The tight patch voltage clamp method is standard for studying individual ion channels in living cells.
  • Ion channel properties are typically identified and categorized based on electrophysiological recordings.
  • It is generally assumed these recordings represent native, undisturbed cell membranes.

Purpose of the Study:

  • To investigate the physical basis of the tight patch voltage clamp method.
  • To determine if single channel recordings accurately reflect ion channels in native cell membranes.
  • To evaluate the potential influence of lipid blebs on electrophysiological measurements.

Main Methods:

  • Utilizing the tight patch voltage clamp technique.
  • Observing the formation of lipid blebs during patch clamp electrode application.

Related Experiment Videos

  • Analyzing the biophysical properties of ion channels in relation to membrane structures.

    • Main Results:

    • Suction applied to patch clamp electrodes readily induces the formation of lipid blebs.
    • These observations suggest that many recorded single ion channels are located within these lipid blebs.
    • The integrity of the native membrane during recording is questionable.

    Conclusions:

    • The physical basis of the tight patch voltage clamp method requires re-evaluation.
    • Single channel recordings may not always represent ion channels in undisturbed native membranes.
    • Lipid blebs are a significant factor to consider in interpreting patch clamp electrophysiology data.