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Updated: Jul 19, 2026
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
Characterization of estrogen receptors alpha and beta in uterine leiomyoma cells
Francisco Valladares1, Ignacio Frías, Delia Báez
1Departamento de Anatomía, Anatomía Patológica e Histología, Universidad de La Laguna, Tenerife, Spain.
Objective:
Cellular and subcellular localization of estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) in uterine leiomyomas.
Design:
Retrospective study.
Setting:
University of La Laguna (ULL) and Canary University Hospital (HUC).
Patient(S):
Premenopausal and postmenopausal women with uterine leiomyomas.
Intervention(S):
Hysterectomy and myomectomy.
Result(S):
Estrogen receptor alpha was only present in smooth muscle cells with variation in the subcellular location in different leiomyomas. Estrogen receptor beta was widely distributed in smooth muscle, endothelial, and connective tissue cells with nuclear location in all cases studied; variations were only found in the muscle cells for this receptor.
Conclusion(S):
Estrogens operate in leiomyoma smooth muscle cells through different receptors, alpha and beta. However they only act through the ERbeta in endothelial and connective cells.
