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Published on: August 29, 2012
In vivo effects of recombinant granulocyte-colony stimulating factor in athymic nude mice
1Division of Radiation Hazards, National Institute of Radiological Sciences, Chiba, Japan.
The possible role of T lymphocytes in in vivo regulation of haemopoiesis by recombinant human granulocyte-colony stimulating factor (rhG-CSF) was evaluated. Athymic nude (nu/nu) mice and their normal (+/+) littermates were injected subcutaneously twice daily with 100 micrograms/kg per day of rhG-CSF for 5 days. Such parameters as number of neutrophils in blood, spleen weight and cellularity, bone marrow cellularity, and number of stem and progenitor cells (colony forming units in spleen [CFU-S], mix colony forming cells [Mix-CFC], granulocyte-macrophage colony forming cells [GM-CFC] in bone marrow and spleen were evaluated. Some effects of the rhG-CSF treatment were similar in nu/nu and +/+ mice. Others, however, were to some extent different in the two groups of animals. It is concluded that T lymphocytes may be partially responsible for some of the effects of rhG-CSF in vivo activity.
The possible role of T lymphocytes in in vivo regulation of haemopoiesis by recombinant human granulocyte-colony stimulating factor (rhG-CSF) was evaluated. Athymic nude (nu/nu) mice and their normal (+/+) littermates were injected subcutaneously twice daily with 100 micrograms/kg per day of rhG-CSF for 5 days. Such parameters as number of neutrophils in blood, spleen weight and cellularity, bone marrow cellularity, and number of stem and progenitor cells (colony forming units in spleen [CFU-S], mix colony forming cells [Mix-CFC], granulocyte-macrophage colony forming cells [GM-CFC] in bone marrow and spleen were evaluated. Some effects of the rhG-CSF treatment were similar in nu/nu and +/+ mice. Others, however, were to some extent different in the two groups of animals. It is concluded that T lymphocytes may be partially responsible for some of the effects of rhG-CSF in vivo activity.
