Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Self-Efficacy01:29

Self-Efficacy

234
Self-efficacy is the belief in one's capacity to organize and execute actions necessary to manage prospective situations. This belief significantly influences how individuals approach goals, tasks, and challenges across different domains of life.Psychological and Educational ImpactsIndividuals with strong self-efficacy are more resilient in the face of difficulties. They are more likely to adopt effective problem-solving strategies, persist through obstacles, and regulate emotions such as...
234
Reproductive Cloning01:27

Reproductive Cloning

32.8K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
32.8K
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

2.0K
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
2.0K
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

7.6K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
7.6K
Response Surface Methodology01:16

Response Surface Methodology

674
Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
674
Systematic Error: Methodological and Sampling Errors01:15

Systematic Error: Methodological and Sampling Errors

11.0K
In the case of systematic errors, the sources can be identified, and the errors can be subsequently minimized by addressing these sources. According to the source, systematic errors can be divided into sampling, instrumental, methodological, and personal errors.
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

True <i>Schistosoma mansoni</i> eggs-cretome revealed by laser microdissection of infected mouse liver and intestine.

Frontiers in cellular and infection microbiology·2026
Same author

Design of electronically tunable fractional-order elements based on distributed MOS transistor structures.

Scientific reports·2026
Same author

Functional characterization of secreted Schistosoma mansoni serpin SmSPI reveals factor XIIa inhibition as evidenced by biochemical and human plasma assays and supported by AI-assisted structural modeling.

International journal for parasitology·2026
Same author

Overset-Grid Method with Smooth Orbital Partitioning for Molecular Scattering Calculations.

Journal of chemical theory and computation·2025
Same author

Probing autoionization decay lifetimes of the 4d-16ℓ core-excited states in xenon using attosecond noncollinear four-wave-mixing spectroscopy.

The Journal of chemical physics·2025
Same author

Pan-genome and haplotype map of cassava cultivars and wild ancestors provide insights into its adaptive evolution and domestication.

Molecular plant·2025
Same journal

Integrated RNA-seq and RNAi analyses reveal that ABCF2 is involved in defense against Vibrio parahaemolyticus in Penaeus vannamei.

Genomics·2026
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
06:28

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

836

Efficacy of clone fingerprinting methodologies.

William M Nelson1, Jan Dvorak, Ming-Cheng Luo

  • 1Arizona Genomics Computational Laboratory, BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.

Genomics
|October 3, 2006
PubMed
Summary
This summary is machine-generated.

Comparing fingerprinting methods for bacterial artificial chromosome (BAC) physical maps, this study found the five-enzyme SNaPshot technique most effective. Higher coverage beyond 10x significantly improved assembly, contrasting previous findings.

More Related Videos

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.1K
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA
09:39

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

Published on: July 15, 2011

27.8K

Related Experiment Videos

Last Updated: Feb 10, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
06:28

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

836
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.1K
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA
09:39

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

Published on: July 15, 2011

27.8K

Area of Science:

  • Genomics
  • Bioinformatics
  • Computational Biology

Background:

  • Physical map construction is crucial for genome sequencing and analysis.
  • High-information content fingerprinting techniques accelerate BAC-based physical map creation.
  • Evaluating different fingerprinting methodologies is essential for optimizing map construction.

Purpose of the Study:

  • To compare the effectiveness of various fingerprinting techniques for BAC-based physical map construction.
  • To investigate the impact of fingerprinting coverage on physical map assembly quality.
  • To provide simulation-based evidence for selecting optimal methods and coverage levels.

Main Methods:

  • Extensive simulation of fingerprinting data.
  • Evaluation of multiple enzyme-based fingerprinting techniques, including SNaPshot.
  • Analysis of assembly quality across different fingerprinting coverages (e.g., >10x).

Main Results:

  • The five-enzyme SNaPshot method demonstrated superior performance compared to other evaluated techniques.
  • Increased fingerprinting coverage beyond 10x significantly enhanced physical map assembly.
  • Simulation results contradicted previous findings suggesting a plateau in assembly improvement at 10x coverage.

Conclusions:

  • The SNaPshot technique is highly effective for BAC-based physical map construction.
  • Maximizing fingerprinting coverage beyond 10x is beneficial for improving map assembly.
  • Simulation studies provide valuable insights for optimizing genomic mapping strategies.