Related Experiment Video
Updated: Jul 19, 2026
07:27
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
Published on: April 9, 2021
Proteomic identification of palmitoylated proteins
Amy F Roth1, Junmei Wan, William N Green
1Department of Pharmacology, Wayne State University School of Medicine, Elliman Building, Room 1224, 421 E. Canfield, Detroit, MI 48201, USA.
Methods (San Diego, Calif.)
|October 3, 2006
Summary
A new method purifies and identifies palmitoylated proteins using fatty acid exchange labeling and biotinylation. This technique enables the specific isolation and mass spectrometry analysis of palmitoylated proteins from complex samples.
Area of Science:
- Biochemistry
- Proteomics
- Molecular Biology
Background:
- Palmitoylation is a crucial post-translational modification regulating protein function.
- Identifying palmitoylated proteins in complex biological samples remains challenging.
Purpose of the Study:
- To describe a novel proteomic method for the purification and identification of palmitoylated proteins.
- To evaluate the utility and limitations of this method.
Main Methods:
- Utilizes fatty acid exchange labeling chemistry to attach biotinylated compounds to palmitoylated proteins.
- Employs affinity purification to isolate modified proteins from complex extracts.
- Identifies purified proteins using mass spectrometry.
Main Results:
- Successfully purified and identified a subset of palmitoylated proteins.
- Demonstrated the applicability of the method in yeast (Saccharomyces cerevisiae).
Conclusions:
- The described method provides a powerful tool for studying protein palmitoylation.
- Offers advantages for identifying palmitoylated proteins but requires careful consideration of potential pitfalls.
Related Concept Videos
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:33Peptide Identification Using Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...