Related Experiment Video

Updated: Jul 5, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Silencing stathmin gene expression by survivin promoter-driven siRNA vector to reverse malignant phenotype of tumor

Hui-Zhong Zhang1, Yan Wang, Ping Gao

  • 1Department of Clinical Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. huizhong@public.xa.sn.cn <huizhong@public.xa.sn.cn>

Cancer Biology & Therapy
|October 3, 2006
PubMed

Insights

This study developed a survivin promoter-driven siRNA vector to target the stathmin gene in tumor cells. This approach selectively silenced stathmin, inhibiting tumor growth and inducing apoptosis, showing potential for targeted gene therapy.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Therapy

Background:

  • Stathmin gene overexpression is crucial for tumor cell malignancy.
  • Targeting stathmin offers potential for cancer therapeutics.
  • Ensuring tumor specificity is key for clinical applications.

Purpose of the Study:

  • To design a survivin promoter-driven siRNA vector targeting the stathmin gene.
  • To evaluate the vector's efficacy in selectively silencing stathmin in tumor cells.
  • To assess the therapeutic potential of this targeted gene silencing approach.

Main Methods:

  • Constructed a survivin promoter-driven siRNA eukaryotic expression vector targeting stathmin.
  • Transfected human cervical cancer (Hela) and osteosarcoma (SSOP-9607) cells, using ECV304 cells as a control.
  • Analyzed stathmin expression, cell growth, cell cycle (Flow Cytometry), and apoptosis (TURNEL staining).

Main Results:

  • Selective knockdown of stathmin expression in Hela and SSOP-9607 cells.
  • Significant inhibition of tumor cell growth compared to controls.
  • Induction of G2/M phase cell cycle arrest and increased apoptosis in transfected tumor cells.

Conclusions:

  • Survivin promoter-driven stathmin siRNA vector effectively silences stathmin in tumor cells.
  • This targeted gene silencing demonstrates significant anti-tumor effects.
  • The developed vector shows promise for targeted tumor gene therapy.

More Related Videos

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures07:48

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Published on: December 26, 2016

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...